General information
Mammalian cell lines (BHK, CHO, HEK 293 etc.) are widely used for the production of recombinant glycoproteins, e.g. vaccines, enzymes as well as hormones and immunobiologicals (antibodies, interleukins, lymphokines).
Test Phase (please refer to Table 1)
- Transfection of mammalian cells
- Transient protein production for fast generation of small protein amounts
- Cell harvest and lysate preparation or supernatant
- Strep-Tactin® and/or Ni-NTA affinity purification, SDS-PAGE, Western blot
- Estimation of the required culture volume to obtain the requested amount
of protein
- Time required: approximately 2 weeks
Process development on request
- Adaption to serum-/protein-free media for the production of biopharmaceuticals
- Optimization of freezing conditions in serum-free media
- Optimization of cultivation conditions: CO2 supply, glutamine/GlutaMAX media
- Analytics as required by the customer: e.g. cell number and viability,
glucose/lactate, amino acid analysis, pH measurement, ammonium determination
Production Phase (please refer to Table 1)
- Transient transfection of mammalian cells is a powerful technology for the fast production of small amounts of recombinant proteins. Depending on adherent or suspension cells the customer can choose between 0,25 m2 to 1 m2 corresponding to 1L to 5L cultures.
- Strep-Tactin® and/or Ni-NTA affinity purification
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