IBA : Custom Services

Order Information

These services comprise the precise cloning of the desired gene into IBA’s proprietary StarGate^® Entry Vectors and subsequently the transfer into the StarGate^® Acceptor Vectors with optimal control of expression in E. coli, yeast, insect or mammalian cells.

General procedure

Consulting and planning of the cloning strategy
Primer synthesis and PCR with proof-reading DNA polymerase (if necessary)
Alternatively, de novo synthesis of the desired gene
Entry Cloning into the Entry Vector (basis for all further transfer reactions)
Transfer Cloning into customer-defined Acceptor Vector(s)
Control sequencing of insert

Entry Cloning (Generation of Donor Vector)

Depending on the gene template provided by the customer, two different Entry Cloning procedures can be performed:

Entry Cloning using gene from gene synthesis

The desired gene is chemically synthesized and cloned into the ENTRY Vector. Codon optimization is performed upon request.

Entry Cloning using PCR from plasmid DNA

The gene of interest (GOI) is amplified by PCR from a plasmid DNA template and is inserted precisely into the ENTRY Vector.

Additional costs for sequencing will be charged per base pair depending on the gene length.

› Time required: 4-8 weeks (depending on the cloning procedure)

Cloning is planned in close cooperation with the client. For example, we can introduce specific point mutations (such as additional restriction sites) into the termini of a gene via PCR cloning.

The original control sequencing print-outs of the gene insert can be provided on request. IBA does not guarantee that the encoded gene will be expressed.

Transfer Cloning into Acceptor Vectors (Generation of Destination Vectors)

After sequence confirmation, the resulting Donor Vector is the origin for highly parallel subcloning of your gene of interest into a multitude of Acceptor Vectors, each providing different functional elements like host specific promoters and a variety of purification tags. Subsequently, the resulting Destination Vectors are transformed into the corresponding host cells for protein expression.

Please download the up-to-date list of StarGate^® Acceptor Vectors here.

For adaptation of your vectors into StarGate^® Acceptor Vectors please contact our experts at info@iba-go.com. We are looking forward to discussing your project.

Cloning into IBA´s Classic Vectors

Standard cloning and expression is based on the StarGate^® system. IBA´s classic cloning vectors are used on request.


	Tailor-made Acceptor Vectors are available on request: info@iba-go.com

Order information
see also "how to order"

product	cat. no.	order & prices
Subcloning using restriction enzymes	2-2000-003
Cloning using PCR from plasmid DNA	2-2000-001
Cloning using PCR from plasmid DNA; each additional if orders > 1	2-2000-011
Cloning using PCR from cDNA bank or genomic DNA	2-2000-004
Additional costs for cloning using PCR, per bp	2-2000-002
ENTRY-Cloning via Gene Synthesis	2-2002-001
ENTRY-Cloning via Gene Synthesis; each additional if orders > 1	2-2002-011
ENTRY-Cloning via Gene Synthesis, additional costs per bp < 2kb	2-2002-002
ENTRY-Cloning via Gene Synthesis, additional costs per bp < 5kb	2-2002-003
ENTRY-Cloning via Gene Synthesis, additional costs per bp > 5kb	2-2002-004
Transfer cloning	2-2003-001
Transfer cloning; each additional if orders > 1	2-2003-011

For difficult gene syntheses prices and delivery times are adapted accordingly;
IBA is not obliged to accept difficult gene syntheses.

StarGate® Combinatorial Cloning

Cloning into IBA´s Classic Vectors

StarGate^® Combinatorial Cloning