IBA : NAPS

Several new applications have recently been described in the literature involving chimeric oligonucleotides (e.g. 1-4). Responding to the increasing demand of our customers we are offering a wide variety of combinations (e.g. DNA/RNA, RNA/2'-O-Me-RNA, 2'-F-RNA/DNA). Our extensive range of fluorescent and non-fluorescent markers can of course be incorporated as well.

Features

Chemically synthesized
Specific labeling with fluorescent markers and other non-radioactive reporter groups
Easy introduction of modified bases
Wide variety of combinations available

Possible combinations
	DNA	RNA	2'-O-Me- RNA	2'-F-RNA	2'-NH₂-RNA
DNA		x	x	x	x
RNA	x		x	x	x
2'-O-Me- RNA	x	x		x	x
2'-F-RNA	x	x	x		x
2'-NH₂-RNA	x	x	x	x

References

1) Wu XS, Liu DP, Liang CC, 2001: Prospects of chimeric RNA-DNA oligonucleotides in gene therapy. J Biomed Sci, Nov-Dec;8(6):439-45

2) Liu L, Rice MC, Kmiec EB, 2001: In vivo gene repair of point and frameshift mutations directed by chimeric RNA/DNA oligonucleotides and modified single-stranded oligonucleotides. Nucleic Acids Res Oct 15;29(20):4238-50

3) Kmiec EB, Johnson C, May GD, 2001: Chloroplast lysates support directed mutagenesis via modified DNA and chimeric RNA/DNA oligonucleotides. Plant J Aug;27(3):267-74

4) Gamper HB, Parekh H, Rice MC, Bruner M, Youkey H, Kmiec EB, 2000: The DNA strand of chimeric RNA/DNA oligonucleotides can direct gene repair/conversion activity in mammalian and plant cell-free extracts. Nucleic Acids Res, Nov 1;28(21):4332-9.

For quotations for chimeric oligonucleotides please
contact our (nucleic acid department) at
oligo@iba-go.com.

Order information
see also "how to order"

Order online

product	amount	cat.no.	price
DNA	1.00 µmol	5-0110-014	1.65 EUR/base
RNA	1.00 µmol	5-0510-014	12.50 EUR/base
2'-NH₂ -RNA	1.00 µmol	5-0511-014	35.50 EUR/base
2'-F-RNA	1.00 µmol	5-0512-014	35.50 EUR/base
2'-O-Me- RNA	1.00 µmol	5-0513-014	12.50 EUR/base
HPLC grade purification	1.00 µmol	5-1020-004	15.00 EUR/base
PAGE purification	0.05 µmol	5-1060-002	100.00 EUR/oligo
	0.20 µmol	5-1060-003	125.00 EUR/oligo
	1.00 µmol	5-1060-004	150.00 EUR/oligo

2'-NH₂ -RNA , 2'-F-RNA and 2'-O-Me-RNA are only available with HPLC grade purification or PAGE purification. Further scales available. Ask for a specific quote at oligo@iba-go.com.



		Chimeric Oligonucleotides

Chimeric Oligonucleotides