Successfully Transfected Cells

Mammalian and other eukaryotic cells that have been efficiently transfected using MATra

To aquire these data either MATra-A Reagent has been used or a combination of MA Lipofection Enhancer with IBAfect* or IBAfect alone.
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Adherent cells

  MA
Transfection		 MA
Lipofection		 Lipofection
Cell line Cell type/tissue origin Organism MATra-A MA Lipofection
Enhancer with
IBAfect*		 IBAfect
HeLa cervix carcinoma human + + +
HEK 293 kidney human + + +
BHK-21 kidney hamster n.d. + +
CHO ovary hamster + + +
NIH 3T3 fibroblast mouse + + +
Works for the 5 most popular cell lines!
 
MOLT-4 acute lymphoblastic leukemia  human n.d. + n.d.
H295R adrenocortical carcinoma human n.d. + n.d.
16HB140 airway epithelium human + n.d. n.d.
A549 alveolar basal epithelial  human + + n.d.
FL amnion human + n.d. n.d.
U373 astrocytoma human [+] [+] [+]
EJ28 bladder cancer human + n.d. n.d.
HBL-100 breast human n.d. + +
MCF7 breast adenocarcinoma human + + +
MCF7-182R6 breast adenocarcinoma human + n.d. n.d.
MCF7-TAMR1 breast adenocarcinoma human + n.d. n.d.
MCS7 breast adenocarcinoma human + n.d. n.d.
MDA-MB231 breast adenocarcinoma human + n.d. n.d.
NYGM  cerebral glioblastoma  human n.d. + n.d.
HCT 116 colon adenocarcinoma human n.d. + +
HCT 15 colon adenocarcinoma human n.d. + +
LoVo colon adenocarcinoma human + + n.d.
SW480 colon adenocarcinoma human n.d. + +
Colo 320DM colon carcinoma human + n.d. n.d.
BeWo embryonic/extra-embryonic               epithelial: choriocarcinoma human + + n.d.
A431 epidermoid carcinoma human n.d. + +
MRC 5 fetal lung human + + n.d.
HT-1080 fibrosarcoma human + n.d. n.d.
HFF foreskin fibroblast human [+] [+] n.d.
A172 glioblastoma human n.d. + n.d.
LN18 glioblastoma human + + n.d.
YH-13 glioblastoma human n.d. + n.d
LN229 glioblastoma  human + + n.d.
T98G glioblastoma  human n.d. + n.d.
U251 glioblastoma  human n.d. + n.d.
U87 glioblastoma  human n.d. + n.d
YK6-1 glioblastoma  human n.d. + n.d.
HN12  Head, neck carcinoma human  n.d.  +  n.d.
P4R5 HeLa derivative human + n.d. n.d.
TZMBL HeLa derivative human + n.d. n.d.
Hep G2 hepatocellular carcinoma human + + +
HuH7 hepatocellular carcinoma human + n.d. n.d.
CEMx174 hybrid T/B-lymphocytes  human n.d. + n.d.
FaDu hypopharyngeal carcinoma human + n.d. n.d.
293 T kidney human + + +
293 T-17 kidney human n.d. + +
HPBG kidney human + n.d. n.d.
TSA201 kidney human + n.d. n.d.
NCI-H441 lung adenocarcinoma epithelial  human + + n.d.
He99 lung cancer human + + n.d.
A549 lung carcinoma human + + +
SK-MES-1 lung carcinoma human + n.d. n.d.
BEAS-2B  lung epithelial cells human  +  n.d.  n.d.
NCl lung epithelium human [+] [+] n.d.
H9 lymphoblastoid  human  n.d.  +  n.d.
MEP  mammalian epithelial  human n.d. + n.d.
Mel 888 melanoma human + n.d. n.d.
Mel Ei melanoma human + n.d. n.d.
MeWo melanoma human + + +
SK-MEL-28 melanoma human n.d. + +
HMEC-1 microvascular endothelial human + n.d. n.d.
NCI-H292 mucoepidermoid carcinoma human + n.d. n.d.
SHSY-5Y neuroblastoma human + + +
SK-N-SH neuroblastoma human +** +** +**
SK-N-BE2 neuroblastoma  human + n.d. n.d.
BTK-143 osteosarcoma human n.d. + +
SaOS-2 osteosarcoma human n.d. + +
HOS  osteosarcoma  human n.d. + n.d.
CHO  ovarian  human  +  +  n.d.
181RDB pancreatic human n.d. + +
PC-3 prostate carcinoma human + [+] [+]
HSG salivary gland, submandibular human n.d. + +
HaCaT skin/hair epithelial: keratinocyte human + + +
NCI H-82 small cell lung cancer human + n.d. n.d.
NT2-D1 testis carcinoma human + [+] [+]
SV40 transformed fibroblasts human + n.d. n.d.
ECV-304 urinary bladder carcinoma human n.d. + +
 
bTC-tet  B-cell line mouse + + n.d.
OP9 bone marrow-derived stromal  mouse n.d. + n.d.
CT-26 colon carcinoma mouse + +** n.d.
F9 embryonal carcinoma mouse + n.d. n.d.
P19 embryonic epithelium mouse + n.d. n.d.
STC-1  enteroendocrine I cells  mouse  +  +  n.d.
NIH 3T3 fibroblast mouse + + +
MEF  fibroblast  mouse  +  n.d.  n.d.
L929 fibroblast, connective tissue mouse + n.d. n.d.
11.5dpcXY gonad explants  mouse n.d. + n.d.
HPL hippocampal cell mouse + n.d. n.d.
HT-22 hippocampal neuroblast mouse n.d. + +
mIC-(cl2) intestine mouse n.d. + +
FTO liver carcinoma mouse + n.d. n.d.
B16F10 melanoma mouse + n.d. n.d.
B16F10.9 melanoma mouse + n.d. n.d.
mIEnd  Mesenteric lymph node  mouse  n.d.  +  n.d.
P-388D1 monocyte-macrophage mouse [+] [+] [+]
RAW264 monocyte-macrophage, leukemia mouse + n.d. n.d.
SVEC murine cell line  mouse n.d. + n.d.
Hepa1c1c7 murine hepatoma  mouse + n.d. n.d.
C127 murine mammary tumor mouse n.d. + n.d.
C2C12 myoblast mouse + [+] [+]
N2a neuroblastoma mouse + n.d. n.d.
ND7 neuroblastoma mouse + n.d. n.d.
NS20Y neuroblastoma mouse n.d. + +
Cal27 oral adenosquamous carcinoma  mouse n.d. + n.d.
AtT20 pituitary tumor mouse + n.d. n.d.
NSC primary neural stem cells mouse + n.d. n.d.
SM 10 trophoblast mouse n.d. + +
 
PC12 adrenal pheochromocytoma rat + n.d. n.d.
A7r5 aortic smooth muscle rat n.d. + n.d.
PC6-3 daughter cell line of PC12 rat + n.d. n.d.
C6 glioma rat n.d. + +
HNSCC  Head, neck carcinoma rat  n.d.  +  n.d.
5L hepatocellular carcinoma rat [+] [+] [+]
H4IIE hepatoma rat n.d. + +
RIE1-α5  intestinal rat + n.d. n.d.
EMC mesothelium rat n.d. + +
B103 neuroblastoma rat + n.d. n.d.
INS-1E pancreatic beta cell rat + n.d. n.d.
L6 skeletal muscle cell rat [+] [+] n.d.
RV vein rat + n.d. n.d.
 
COS-1 kidney monkey + n.d. n.d.
COS-7 kidney monkey + [+] n.d.
CV-1 kidney monkey + n.d. n.d.
Vero 76 kidney monkey + n.d. n.d.
Vero kidney monkey n.d. + n.d.
 
BHK-21 kidney hamster n.d. + +
V79 lung fibroblast hamster + n.d. n.d.
CHO ovary hamster + + +
CHO (AA8) ovary hamster + n.d. n.d.
CHO-K1 ovary hamster + n.d. n.d.
B11  (UV) resistant ovary  hamster  n.d.  +  n.d.
 
Sf9 (ATCC) pupal ovary fall army worm + n.d. n.d.
PT-11 kidney bovine n.d. + +
MDBK-RI kidney bovine + n.d. n.d.
MDBK-ZH kidney bovine + n.d. n.d.
KOP/R oesophagus bovine + n.d. n.d.
MDCK kidney canine + n.d. n.d.
CRFK kidney cat n.d. + +
CCB brain carp + n.d. n.d.
LHM hepatoma chicken + n.d. n.d.
VSa13 bone (chondrocyte-like) fish + n.d. n.d.
PTK2 kidney potoroo + n.d. n.d.
LLC-PK1 kidney pig + n.d. n.d.
SPEV kidney pig + n.d. n.d.
PK15 kidney pig + n.d. n.d.
AM-C6SC8 kidney pig n.d. + +
RK 13 kidney rabbit + n.d. n.d.
QM9 muscle quail + n.d. n.d.
A6 kidney xenopus + n.d. n.d.
-  fibroblasts  xenopus  +  n.d.  n.d.
EMC  mesenthelial. Cells (epicard)    n.d.  +  n.d.

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Suspension cells

  MA
Transfection		 MA
Lipofection		 Lipofection
Cell line Cell type/tissue origin Organism MATra-A MA Lipofection
Enhancer with
IBAfect*		 IBAfect
THP-1 acute myeloid leukemia human + + +
Jurkat acute T-cell lymphoma human [+] + +
BL-41 B-cell lymphoma human and MATra-S + n.d. n.d.
K562 chronic myeloid lymphoma human and MATra-S + n.d. n.d.
U937 histiocytic lymphoma human and MATra-S + + n.d.
NB-4 myeloid (unspec.)
blood/immune cells		 human + n.d. n.d.
 
P815 mastocytoma mouse n.d. + +
S2 embryonic drosophila [+] [+] [+]
SF9 pupal ovary fall army worm [+] [+] [+]

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Primary cells
  MA
Transfection		 MA
Lipofection		 Lipofection
Cell line Cell type/tissue origin Organism MATra-A MA Lipofection
Enhancer with
IBAfect*		 IBAfect
  adherent gastric cells human n.d. + n.d.
  aortic endothelial cells human +  n.d. n.d.
  B-lymphocyte human and MATra-S + n.d. n.d.
  cardiomyocytes human +  n.d. n.d.
  chondrocytes  human  +  +  n.d.
  colon carcinoma human + n.d. n.d.
  dendritic cell human and MATra-S + n.d. n.d.
  dermal microvascular endothelial cell (HDMEC) human + n.d. +
  human umbilical vein endothelial cells (HUVEC) human +  n.d. n.d.
  endothelial cells (cord blood) human + n.d. n.d.
  fibroblasts human +  +  + 
  fibroblasts, diploid human +  +  + 
  gastric gland  human +  +  n.d.
  gastric myofibroblasts  human  +  n.d.  n.d.
  glioma human + n.d. n.d.
  head and neck cancer cell human +  n.d. n.d.
  hematopoietic progenitor cell human +  +  + 
  hippocampal neurons human + n.d. n.d.
  keratinocytes human n.d. +  + 
  kidney human + n.d. n.d.
  LNCaP human n.d. +  + 
  mammal epithelium human n.d. +  + 
  nasal airway epithelium human +  +  n.d.
  pancreatic cell human n.d. +  n.d.
  pancreatic tumor human + n.d. n.d.
  peripheral blood lymphocytes human +  +  + 
  stroma cells (endometrium) human n.d. +  + 
  T-lymphocyte human + +  n.d.
  trophoblastic cells human n.d. +  + 
  uterus  human + n.d. [+]
 
  adherent gastric cells mouse n.d. + n.d.
  embryonic fibroblasts (MEF) mouse +  +  +
  embryonic stem cell (mES) mouse + n.d. n.d.
  endothelial cell mouse + n.d. n.d.
  endothelial cell, heart mouse + n.d. n.d.
  endothelial cell, lung mouse + n.d. n.d.
  hippocampal neurons mouse +  n.d. n.d.
  macrophage mouse +  n.d. n.d.
  myoblasts mouse + + n.d.
  neuroblastoma mouse + n.d. n.d.
  neurons mouse + + n.d.
  neurons, cortical mouse + n.d. n.d.
  peripheral blood lymphocytes mouse n.d. +  + 
  thymus cell mouse [+] [+] [+]
  T-lymphocyte mouse +  n.d. n.d.
  vagal afferent neurons mouse n.d. + n.d.
 
  aortic smooth muscle cells  rat  n.d.  +  n.d.
  cardiomyocyte  rat n.d. + n.d.
  cortical neurons  rat  n.d.  +  n.d.
  endothelial cell, aorta rat [+] [+] [+]
  hepatocyte rat + + +
  hippocampal neuron rat + [+] [+]
  liver cell rat [+] [+] [+]
  neuron (E18.5) rat + n.d. n.d.
  SG (ganglion) rat [+] [+] n.d.
 
  aortic endothelial cell bovine + n.d. n.d.
  carotid artery smooth muscle bovine + n.d. n.d.
  chromaffine cell bovine n.d. + +
  lens bovine + n.d. n.d.
  nasal airway epithelium bovine + n.d. n.d.
 
  airway epithelium pig n.d. + +
  chondrocyte pig + n.d. n.d.
  fibrochondrocyte pig + n.d. n.d.
  pSM, smooth muscle pig n.d. + +
  testis cell pig +  n.d. n.d.
 
  fibroblast chicken + n.d. n.d.
  synovial cell goat +  n.d. n.d.
  Mamestra brassicae lepidopteran (insect cell) +  n.d. n.d.
  kidney cell monkey +  n.d. n.d.
  thymus cell rabbit +  n.d. n.d.
  thymus cell sheep +  n.d. n.d.
  plexus sheep +  n.d. n.d.
  thymus cell wisent +  n.d. n.d.

* in some examples given in this table a lipofection reagent other than IBAfect has been used
** preliminary data
n.d. = not determined
[+] = either MATra, Enhancer or IBAfect has been used
Last update March 2011

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