MATra Methods

Downloads: MATra Manual (pdf 213 KB) IBAfect Manual (pdf, 75 KB) Trouble Shooting (pdf, 14 KB) Frequently Asked Questions (pdf, 76 KB)

Methods

We recommend using both procedures in parallel (Magnet Assisted Transfection and Magnet Assisted Lipofection) to determine the optimal method for your particular cells of interest. * the loading of MATra-A Reagent with nucleic acids has to be performed under serum-free conditions while the nucleic acids loaded MATra-A Reagent can be applied to the cells in the presence of serum ** any commercially available transfection reagent based on polycations or lipids

Easy protocol (example with MATra-A Reagent)

1. Dilute nucleic acid in medium
   
   
2. Add magnetic nanoparticle (MATra-A Reagent)
   
   
3. Incubate 20 - 30 minutes
   
   
4. Add medium to adherent cells (2 - 4 x 10⁵ cells)
   
   
5. Add nucleic acid/nanoparticle solution
   
   
6. Place culture plate on magnet plate
   
   
7. Incubate 15 minutes
   
   
8. Remove magnet plate

Type of nucleic acid/virus suitable for MATra

• Plasmid DNA
  
• Antisense oligonucleotides
  
• siRNA
  

MA Lipofection Enhancer only:

• Adenovirus
  
• Retrovirus