Inosine and uridine modifications
| Oligonucleotides with inosine for wobble positions: We introduce as many inosines as you require! |
2'-Deoxyinosine (2'-dI)
2'-Deoxyuridine (2'-dU)
5-Fluoro-2'-deoxyuridine (5-F-2'-dU)
5-Iodo-2'-deoxyuridine (5-Iodo-2'-dU)
2-Aminopurine and Deoxypurine
Stable abasic site
 2'-Deoxyinosine (2'-dI)
2´-Deoxyinosine (2´-dI) in an oligonucleotide is used for wobble positions, since it can form base pairs with all bases. As a specialty, we are able to introduce as many inosines as you require into one oligonucleotide! Several experiments have suggested that deoxyinosine might be an "inert" base; its presence in an oligonucleotide sequence seems neither to disturb DNA duplex formation nor to destabilize the duplex. Deoxyinosine containing primers have been used successfully to amplify cDNA or genomic fragments for the generation of DNA probes from a peptide sequence.
Features
- 2´-dI can form base pairs with A, G, C and T*
- useful when parts of your target sequence are unknown
- great for targeting different mutants of a gene
- 50% discount if more than 5 inosine modifications are requested in one order!
* the order of stabilities of base pairings is I-C >> I-A > I-T = I-G
 Order online
| product |
scale |
amount |
cat. no. |
price/coupling |
| 2'-dI |
0.05 µmol |
5 nmol |
5-0234-012 |
18.00 EUR |
| 0.20 µmol |
25 nmol |
5-0234-013 |
20.00 EUR |
| 1.00 µmol |
100 nmol |
5-0234-014 |
35.50 EUR |
Order information
see also "how to order"
A discount of 50 % for each 2'-dI modification is granted if more than 5 modifications are requested in one order, e.g. six 2'-dI in one sequence or one 2-dI in six different sequences.
Structure & Reference
2'-Deoxyuridine (2'-dU)
- Replacement of thymidine with 2'-deoxyuridine results in destabilization of a DNA duplex
- Is used in combination with Uracil-DNA glycosylase to avoid cross-contamination of PCR products
- Useful for cloning of PCR-products (in combination with Uracil-DNA glycosylase)
Order online
| product |
scale |
amount |
cat. no. |
price/coupling |
| 2'-dU |
0.05 µmol |
5 nmol |
5-0235-012 |
18.00 EUR |
| 0.20 µmol |
25 nmol |
5-0235-013 |
20.00 EUR |
| 1.00 µmol |
100 nmol |
5-0235-014 |
35.50 EUR |
Order information
see also "how to order"
A discount of 50 % for each 2'-dU modification is granted if more than 5 modifications are requested in one order, e.g. six 2'-dU in one sequence or one 2'-dU in six sequences.
Structure & Reference
5-Fluoro-2'-deoxyuridine (5-F-2'-dU)
- Halogenated nucleosides are useful for chrystallography
Order online
| product |
scale |
amount |
cat. no. |
price/coupling |
| 5-F-2'-dU |
0.05 µmol |
5 nmol |
5-0235-112 |
55.00 EUR |
| 0.20 µmol |
25 nmol |
5-0235-113 |
79.00 EUR |
| 1.00 µmol |
100 nmol |
5-0235-114 |
95.00 EUR |
Order information
see also "how to order"
Structure
5-Iodo-2'-deoxyuridine (5-Iodo-2'-dU)
- Halogenated nucleosides are useful for chrystallography
Order online
| product |
scale |
amount |
cat. no. |
price/coupling |
| 5-I-2'-dU |
0.05 µmol |
5 nmol |
5-0235-212 |
48.50 EUR |
| 0.20 µmol |
25 nmol |
5-0235-213 |
65.00 EUR |
| 1.00 µmol |
100 nmol |
5-0235-214 |
100.00 EUR |
Order information
see also "how to order"
Structure
2-Aminopurine and Deoxypurine
Order online
| product |
scale |
amount |
cat. no. |
price/coupling |
| 2-AminoPurine |
0.05 µmol |
5 nmol |
5-0230-602 |
55.00 EUR |
| 0.20 µmol |
25 nmol |
5-0230-603 |
79.00 EUR |
| 1.00 µmol |
100 nmol |
5-0230-604 |
95.00 EUR |
| Deoxypurine |
0.05 µmol |
5 nmol |
5-0230-702 |
79.00 EUR |
| 0.20 µmol |
25 nmol |
5-0230-703 |
95.00 EUR |
| 1.00 µmol |
100 nmol |
5-0230-704 |
115.00 EUR |
Order information
see also "how to order"
Structure
Stable abasic site
- One base spacer for oligonucleotides
Order online
| product |
scale |
amount |
cat. no. |
price/coupling |
| Stable abasic site |
0.05 µmol |
5 nmol |
5-0230-612 |
55.00 EUR |
| 0.20 µmol |
25 nmol |
5-0230-613 |
79.00 EUR |
| 1.00 µmol |
100 nmol |
5-0230-614 |
95.00 EUR |
Order information
see also "how to order"
Structure
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