Multiple Cloning Sites

Multiple Cloning Sites (MCS)

General remarks

• Restriction enzymes with two recognition sites are denoted in bold, all other recognition sites mentioned in the MCS are unique
• The overhangs which have to be generated via the PCR strategy (see cloning scheme pASK-IBA3) are indicated in lower case
• BsaI and Eco31I are isoschizomers
• Short linker sequences SerAla or GlyAla are essential in order to ensure steric accessibility of Strep-tag II

Data sheets

For Multiple Cloning Sites please refer to the data sheets below. Features, maps and cloning primers are shown as well.

• pASK-IBA2 map, multiple cloning site (PDF/87 Kb)
  
• pASK-IBA2C map, multiple cloning site (PDF/91 Kb)
• pASK-IBA3C map, multiple cloning site (PDF/95 Kb)
• pASK-IBA3plus map, multiple cloning site (PDF/85 Kb)
• pASK-IBA4 map, multiple cloning site (PDF/90 Kb)
• pASK-IBA4C map, multiple cloning site (PDF/90 Kb)
• pASK-IBA5C map, multiple cloning site (PDF/89 Kb)
• pASK-IBA5plus map, multiple cloning site (PDF/90 Kb)
• pASK-IBA6 map, multiple cloning site (PDF/91 Kb)
• pASK-IBA6C map, multiple cloning site (PDF/92 Kb)
• pASK-IBA7C map, multiple cloning site (PDF/89 Kb)
• pASK-IBA7plus map, multiple cloning site (PDF/90 Kb)
• pASK-IBA12 map, multiple cloning site (PDF/92 Kb)
• pASK-IBA13plus map, multiple cloning site (PDF/91 Kb)
• pASK-IBA14 map, multiple cloning site (PDF/90 Kb)
• pASK-IBA15plus map, multiple cloning site (PDF/95 Kb)
• pASK-IBA16 map, multiple cloning site (PDF/97 Kb)
• pASK-IBA17plus map, multiple cloning site (PDF/96 Kb)
• pASK-IBA32 map, multiple cloning site (PDF/92 Kb)
• pASK-IBA33plus map, multiple cloning site (PDF/86 Kb)
• pASK-IBA35plus map, multiple cloning site (PDF/91 Kb)
• pASK-IBA37plus map, multiple cloning site (PDF/92 Kb)
• pASK-IBA43plus map, multiple cloning site (PDF/98 Kb)
• pASK-IBA44 map, multiple cloning site (PDF/99 Kb)
• pASK-IBA45plus map, multiple cloning site (PDF/99 Kb)
• pASK-IBA63a-plus map, multiple cloning site (PDF/82 Kb)
• pASK-IBA63b-plus map, multiple cloning site (PDF/82 Kb)
• pASK-IBA63c-plus map, multiple cloning site (PDF/82 Kb)
• pASK-IBA65b-plus map, multiple cloning site (PDF/79 Kb)
• pEXPR-IBA3 map, multiple cloning site (PDF/78 Kb)
• pEXPR-IBA5 map, multiple cloning site (PDF/82 Kb)
• pEXPR-IBA7 map, multiple cloning site (PDF/90 Kb)
• pEXPR-IBA13 map, multiple cloning site (PDF/83 Kb)
• pEXPR-IBA15 map, multiple cloning site (PDF/83 Kb)
• pEXPR-IBA17 map, multiple cloning site (PDF/105 Kb)
• pEXPR-IBA42 map, multiple cloning site (PDF/88 Kb)
• pEXPR-IBA43 map, multiple cloning site (PDF/106 Kb)
• pEXPR-IBA44 map, multiple cloning site (PDF/88 Kb)
• pEXPR-IBA45 map, multiple cloning site (PDF/109 Kb)
• pEXPR-IBA103 map, multiple cloning site (PDF/101 Kb)
• pEXPR-IBA105 map, multiple cloning site (PDF/107 Kb)
• pKS1-ST map, multiple cloning site (PDF/96 Kb)
• pKS2-ST map, multiple cloning site (PDF/108 Kb)
• pPR-IBA1 map, multiple cloning site (PDF/77 Kb)
• pPR-IBA2 map, multiple cloning site (PDF/78 Kb)
• pPR-IBA101 map, multiple cloning site (PDF, 99 kb)
• pPR-IBA102 map, multiple cloning site (PDF, 107 kb)