Buffers and reagents for Strep-tag^®

Reagents used in combination with the Strep-tag technology

Of special interest to Strep-tag® users!! IBA-lyse Bacterial Lysis Buffer

The new IBA-lyse Bacterial Lysis Buffer is optimized for preparation of a cleared lysate after cytoplasmic expression of Strep-tag fusion proteins. The buffer is formulated for gentle disruption of E. coli and release of proteins. It consists of a reagent mix compatible with subsequent Strep-tag purification. The protocol provides a simple, rapid, inexpensive and most importantly more reproducible and milder alternative to mechanical methods such as sonication or French Press for preparation of cleared lysates to be submitted to affinity chromatography. In addition, it avoids unwanted heating which occurs with e.g. sonication. Avidin, which is included in the buffer, blocks biotin contaminations which could damage the Strep-Tactin purification columns or cause background on Western blots.

• Buffer B which contains 1 mM EDTA, Tween 20 and avidin
  
• Lyophilized lysozyme
  
• Lysozyme reconstitution buffer
  
• Lyophilized DNase I
  
• DNase reconstitution buffer

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product	amount	cat. no.	order & prices
IBA-lyse Bacterial Lysis Buffer	50 ml*	2-1017-050
IBA-lyse Bacterial Lysis Buffer	250 ml	2-1017-250

Buffer L - Lysis buffer for mammalian cell preparation

Lysis buffer for preparation of cell extracts of mammalian cells.

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product	amount	cat. no.	order & prices
Buffer L, Lysis Buffer for mammalian cells)	100 ml 5x buffer	2-1020-100

Desthiobiotin: Reagent for Strep-tag® II Elution

Protocols for download

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product	amount	cat. no.	order & prices
D-Desthiobiotin solution = 10 x Buffer E*	25 ml 10 x buffer for 250 ml	2-1000-025
D-Desthiobiotin	0.107 g for 200 ml 2.5 mM buffer	2-1000-001
D-Desthiobiotin	1g	2-1000-002
D-Desthiobiotin	5g	2-1000-005

Buffer BE - Biotin Elution Buffer

Elution buffer for competitive displacement of purified recombinant Strep-tag^® or One-STrEP-tag^® fusion proteins from immobilized Strep-Tactin.
This buffer is especially recommended for use with One-STrEP-tag in protein complex or PPI applications since it contains Biotin which has a higher affinity to Strep-Tactin and therefore more efficiently competes with the (One)Strep-tag binding. The regeneration of the Strep-Tactin columns in PPI applications is not recommended to avoid the isolation of cross contaminations. The buffer is also recommended for the elution of MagStrep beads and Strep-Tactin Spin columns.

Please note that after elution with buffer BE the resin cannot be regenerated.

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product	amount	cat. no.	order & prices
Buffer BE, Biotin Elution Buffer	25 ml 10 x buffer	2-1019-025

Strep-tag^® Regeneration Buffer with HABA

Contains HABA (2-[4'-hydroxy-benzeneazo]benzoic acid) which displaces desthiobiotin at the biotin-binding site regenerating Strep-Tactin. The yellow HABA solution turns red when desthiobiotin has been removed from Strep-Tactin clearly indicating the regeneration process and activity status of the column.

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product	amount	cat. no.	order & prices
Strep-tag regeneration buffer with HABA (10 x concentrated)	100 ml 10 x Buffer R	2-1002-100

Strep-tag^® Protein Purification Buffer Set

Convenient set for the purification of recombinant Strep-tag fusion proteins on immobilized Strep-Tactin and for the regeneration of the columns.

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product	amount	cat. no.	order & prices
Strep-tag protein purification buffer set (10 x concentrated buffers)	100 ml 10x Buffer W (W=washing); 25 ml 10x Buffer E (E=elution); 100 ml 10x Buffer R (R=regeneration)	2-1002-001

Biotin Blocking Buffer

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product	amount	cat. no.	order & prices
Biotin Blocking Buffer	2 ml	2-0501-002

Strep-tag^® Washing Buffer

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product	amount	cat. no.	order & prices
Strep-tag washing buffer	100 ml	2-1003-100

Anhydrotetracycline

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product	amount	cat. no.	order & prices
Anhydrotetracycline	25 mg	2-0401-002
Anhydrotetracycline	50 mg	2-0401-001

Avidin

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product	amount	cat. no.	order & prices
Avidin	15 mg	2-0204-015
Avidin	50 mg	2-0204-050

Strep-tag II Peptide

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product	amount	cat. no.	order & prices
Strep-tag II Peptide	1.8 mg	2-1018-002
Strep-tag II Peptide	5 x 1.8 mg	2-1018-009