Ni-NTA Sepharose® resin and columns


For purification of 6xHistidine-tag proteins by gravity flow chromatography

Ni-NTA Sepharose offers high binding capacity and minimal non-specific binding. This material has excellent handling properties for most scales of batch and column purification. Pre-packed columns are available in different formats (1 and 5 ml bed volume).

Specifications Ni-NTA Sepharose
Binding capacity 5-10 mg/ml (300-500 nmol of a 20 kDa protein)
Support Sepharose CL-6B
Bead structure Cross-linked, 6% agarose
Bead size 45-165 µm
Maximal pressure 2.8 psi
Form (bulk) 50% suspension in 30% ethanol, pre-charged with Ni2+


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Order information
see also "how to order"


product amount cat. no. order & prices
Ni-NTA Sepharose gravity flow column (1 ml bed volume) 1 column 2-3202-001 online order
Ni-NTA Sepharose gravity flow column (5 ml bed volume) 1 column 2-3202-051  online order
Ni-NTA Sepharose 20 ml 50 % suspension
(= 10 ml column bed volume)
2-3201-010  online order

Ni-NTA resin is manufactured by Qiagen
Please note, that these products are not available in Japan due to patent restrictions.


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