Ni-NTA system

Ni-NTA Sepharose

Ni-NTA Superflow

Cloning

Detection

For purification of 6xHistidine-tag proteins by gravity flow chromatography Ni-NTA Sepharose offers high binding capacity and minimal non-specific binding. This material has excellent handling properties for most scales of batch and column purification. Pre-packed columns are available in different formats (1 and 5 ml bed volume). Specifications Ni-NTA Sepharose Binding capacity 5-10 mg/ml (300-500 nmol of a 20 kDa protein) Support Sepharose CL-6B Bead structure Cross-linked, 6% agarose Bead size 45-165 µm Maximal pressure 2.8 psi Form (bulk) 50% suspension in 30% ethanol, pre-charged with Ni2+ Need buying or technical advice? Contact a protein expert! +49(0)551 50672-0 Online E-mail (please indicate your complete address) Order information see also "how to order" product amount cat. no. order & prices Ni-NTA Sepharose gravity flow column (1 ml bed volume) 1 column 2-3202-001 Ni-NTA Sepharose gravity flow column (5 ml bed volume) 1 column 2-3202-051   Ni-NTA Sepharose 20 ml 50 % suspension (= 10 ml column bed volume) 2-3201-010   Ni-NTA resin is manufactured by Qiagen Please note, that these products are not available in Japan due to patent restrictions.

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