IBA : Protein Tools

For purification of 6xHistidine-tag proteins by gravity flow chromatography

Ni-NTA Sepharose offers high binding capacity and minimal non-specific binding. This material has excellent handling properties for most scales of batch and column purification. Pre-packed columns are available in different formats (1 and 5 ml bed volume).

Specifications Ni-NTA Sepharose
Binding capacity:	Up to 50 mg/ml (up to 2500 nmol @ ~20 kDa)
Support:	Sepharose CL-6B
Bead structure:	Cross-linked, 6% agarose
Bead size:	45-165 µm
Form:	50% suspension in 30% ethanol, precharged with Ni²⁺

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Order information
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product	amount	cat. no.	order & prices
Ni-NTA Sepharose gravity flow column (1 ml bed volume)	1 column	2-3202-001
Ni-NTA Sepharose gravity flow column (5 ml bed volume)	1 column	2-3202-051
Ni-NTA Sepharose	20 ml 50 % suspension (= 10 ml column bed volume)	2-3201-010

Ni-NTA resin is manufactured by Qiagen
Please note, that these products are not available in Japan due to patent restrictions.

Ni-NTA Sepharose® resin and columns

For purification of 6xHistidine-tag proteins by gravity flow chromatography

Ni-NTA Sepharose^® resin and columns