Detection of 6xHistidine-tag
Primary antibody against 6xHistidine-tag: Mouse anti 6xHistidine mAb, lyophilized |
| Description |
Anti 6xHistidine-tag monoclonal antibody; purified by affinity chromatography |
| Subclass |
IgG1 |
| Specificity |
6xHistidine-tag; the antibody was produced in mouse using 6xHistidine-tag linked to PAX6 transcription factor as immunogen |
| Detection |
N- or C-terminal or internal 6xHistidine-tag |
| Form |
Lyophilized from NH4HCO3, contains 10 mg sucrose per mg protein |
| Reconstitution |
Reconstitute in phosphate buffered saline (PBS) at a concentration of 0.2 – 0.1 mg/ml (i. e. dissolve with
0.5 – 1.0 ml PBS, respectively). Aliquot and store at -20 °C. |
| Dilutions |
Recommended dilution for detection of 6xHistidine-tag fusion proteins in Western blots (chromogenic detection):
- 100-200 ng/ml diluted in PBS containing 0.2% BSA
- Secondary antibody: rabbit anti-mouse pAb, HRP conjugated; Cat. No. 2 1591-001
|
| Amount |
100 µg; sufficient to develop 50-100 Western Blots using
10 ml incubation volume each |
| Stability |
12 months after shipping |
| Storage |
-20 °C |
| Shipment |
RT |
|
Secondary antibody for detection of primary mouse antibody (against 6xHistidine-tagged
(or Strep-tagged)) proteins: Rabbit anti Mouse pAb, HRP conjugated.
| Description |
Purified polyclonal rabbit anti mouse immunoglobulin fraction conjugated to horseradish peroxidase (HRP). |
| Specificity |
The antibody used for conjugation has not been absorbed to remove cross-reactions to immunoglobulin from other species. Therefore the conjugate shows reactions with mouse immunoglobulin of all classes |
| Form |
Dissolved in 50 mM Tris/HCl 15 mM NaN3, pH 7.2 |
| Concentration of conjugate |
1.3 mg/ml |
| Dilutions |
- Dilute 1:1000 for chromogenic detection of bound mouse monoclonal antibodies in Western Blots
- Dilute 1:2000 (or titrate up to 1:10,000) for detection of mouse monoclonal antibodies in microplate assays
|
| Amount |
0.5 ml |
| Stability |
6 months after shipping |
| Storage |
2 - 8 °C |
| Shipment |
RT |
|
Order information
see also "how to order"
| product |
amount |
cat. no. |
order & prices |
| Mouse anti 6xHistidine mAb |
100 µg, for 50 – 100 Western blots |
2-1590-000 |
 |
| Rabbit anti Mouse pAb, HRP conjugated |
0.5 ml |
2-1591-001 |
|
|
