Technology
The Strep-tag story
Principle & properties

Cloning/gene expression

Purification
Introduction
Applications
Resin Specifications
Strep-Tactin Sepharose
Strep-Tactin Superflow
Strep-Tactin MacroPrep
Strep-Tactin POROS
MagStrep
Column formats
Spin columns
High-throughput
 purification
Antibody columns
Buffers & Reagents
Strep-tag Starter Kits

Detection
Introduction
Strep-Tactin HRP
Strep-Tactin AP
Antibodies
Fluorescence detection
Protein ladder

Diagnostic/Assay/
Immobilization

Examples

References

FAQ

for a fast detection of Strep-tag II proteins with conjugates in Western blots The Strep-tag HRP Detection Kit Chemiluminescence Detection Chromogenic Detection Chemiluminescence detection For chemiluminescence detection of Strep-tag proteins on Western blots we offer Strep-Tactin horseradish peroxidase (HRP) conjugate (2-1502-001) for direct detection without the need of a secondary antibody. For details please refer to the Western blot protocol. Specifications of Strep-Tactin HRP conjugate Detection N- or C-terminal or internal Strep-tag II Sensitivity in dot blots > 7 pg/dot Sensitivity in Western blots > 7 pg/band Cross reactivity Reacts with biotin carboxyl carrier protein (22.5 kDa) in E. coli extracts. Generally, biotinylated proteins can be sufficiently masked by a 10 minute preincubation with Biotin Blocking Buffer. Form Suspended in 0.01 M phospate buffered saline pH 7.4 containing BSA and 0.01 % Thimerosal as preservative. Can be diluted 1:10,000 for detection of Strep-tag II fusion proteins bound to microplates or 1:4,000 for detection of Strep-tag II fusion proteins after Western blotting. Stability 1 year Storage 4 °C Shipment RT Chromogenic detection For chromogenic detection of Strep-tag proteins on Western blots we offer Strep-Tactin horseradish peroxidase (HRP) conjugate (2-1502-001) for direct detection without the need of a secondary antibody. For details please refer to the Western blot protocol. Ready-to-use Strep-tag HRP Detection Kit (2-1502-000) (detailed protocol included): The Strep-tag HRP Detection Kit includes all buffers and reagents required for blocking, washing and the chromogenic reaction on a total of 1000 cm2 membrane. Strep-tag Protein Ladder is provided as positive control protein within the HRP Detection Kit and can also be purchased separately (#2-1011-100 sufficient for 100 Applications) Specifications of Strep-tag HRP Detection Kit (sufficient for 1000 cm2 membrane) Component Description Amount Control protein Strep-tag Protein Ladder for 20 lanes Buffer SI Blocking and incubation buffer 10x 50 ml Buffer W Washing buffer 20x 2 x 50 ml Strep-Tactin HRP conjugate at least 2 mol HRP / mol Strep-Tactin 0.125 ml Buffer ER HRP reaction buffer 10x 25 ml HRP substrate 30% H2O2 0.5 ml Chromogen 3% 4-chloro naphtol in methanol 3.0 ml Need buying or technical advice? Contact a protein expert! +49(0)551 50672-0 Online E-mail (please indicate your complete address) Order information see also "how to order" product amount cat. no. order & prices Strep-tag HRP Detection Kit 1 kit 2-1502-000 Strep-Tactin HRP conjugate 0.5 ml 2-1502-001 Strep-tag Protein Ladder (lyophilized) 240 µg; 100 applications 2-1011-100

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