IBA : Protein Tools

for purification of Strep-tag^® II proteins by FPLC or HPLC

Strep-Tactin POROS optimally combines high flow rates with selective binding and high dynamic capacity (see figures below). This material can be used for FPLC or HPLC applications allowing fast Strep-tag protein purification.¹ Strep-Tactin POROS 20 has a bead size of 20 µm, while POROS 50 has a bead size of 50 µm. The binding capacity is identical.
Since the Strep-Tactin resins are optimized for column affinity chromatography as opposed to batch purification, we do recommend our pre-packed 1.7 ml Strep-Tactin POROS columns for FPLC and HPLC. For packing individual columns bulk material is available.

Semi-preparative purification within minutes

Pre-packed column with Strep-Tactin POROS and accessories.

The green fluorescent protein (GFP) with C-terminal Strep-tag II was expressed in the cytoplasm of E. coli. After sterile filtration the crude lysate was separated via Perfusion Chromatography^® on Strep-Tactin POROS 20 (5 mg/ml) under physiological conditions (100 mM Tris-Cl pH 8.0, 1 mM EDTA). Elution was performed by the addition of 5 mM desthiobiotin in the same buffer and chromatography was monitored by measuring the absorbance of the eluate at 280 nm.

The purification is documented on a coomassie stained SDS gel where samples from the crude lysate (lane 1) and from the elution peak at 6.7 minutes (lane 2) had been applied. 3 mg GFP could be obtained in one step when running a 1.7 ml column on a BioCAD^® work-station.

Specifications of Strep-Tactin POROS resin

Binding capacity	1.7 ml column can be used for the one-step purification of 40 to 80 nmol recombinant protein
Support	POROS 20 / POROS 50
Bead size	20 µm / 50 µm
Linear flow rate	300 - 500 cm/h is possible for Strep-tag purification
Form (bulk)	50 % suspension in 100 mM Tris/HCl pH 8.0, 1mM EDTA, 150 mM NaCl
Stability	at least 6 months after shipment
Storage	4 °C, DO NOT FREEZE
Shipment	RT

View more information on the Strep-tag purification system.

Protocols for download
For buffers & reagents pease click here

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Order information
see also "how to order"

product	amount	cat. no.	order & prices
Ready-to-use Strep-Tactin POROS 20 column (1.7 ml bed volume)	1 column	2-1203-017
Ready-to-use Strep-Tactin POROS 50 column (1.7 ml bed volume)	1 column	2-1205-017
Strep-Tactin POROS 20 (50 % suspension)	2 ml	2-1203-001
	4 ml	2-1203-002
	10 ml	2-1203-005
	20 ml	2-1203-010
Strep-Tactin POROS 50 (50% suspension)	2 ml	2-1205-001
	4 ml	2-1205-002
	10 ml	2-1205-005
	20 ml	2-1205-010

¹Please note, that the binding capacity is just about half of that of other matrices as described on Strep-Tactin Sepharose, Superflow and MacroPrep. POROS is not recommended for membrane proteins.



		Strep-Tactin^® POROS^®

Strep-Tactin® POROS®

for purification of Strep-tag® II proteins by FPLC or HPLC

Strep-Tactin^® POROS^®

for purification of Strep-tag^® II proteins by FPLC or HPLC