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This novel cloning system is the perfect tool for efficient screening and fast identification of the optimal tag for PPI investigation with a given bait. Once the bait protein is cloned into the Donor Vector, a large selection of Acceptor Vectors for its expression with different tag arrangements is available.
For PPI analysis we recommend to start with Acceptor Vectors equipped with the following features:
- One-STrEP-tag at N- and C-terminus (for One-STrEP, One-TAP, SPINE)
- One-STrEP/ FLAG®-tag double tag, N- and C-terminal (for Two-TAP)
- Strep-tag®II at N- and C-terminus (for SPINE)
Please refer to the list of Acceptor Vectors for a complete overview of all options currently available.
The recommended Acceptor Vectors are included in the PPI kits (see "Overview of Sets, Kits and Products for PPI") but can also be purchased separately.
For detailed information on the StarGate® TAGnology, please visit our website at www.stargate-cloning.com
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