Catalogue Number: RB21-03
- The Rapid Canine Brucella Ab Test Kit is available from Genprice in the US and Gentaur in Europe.
- This 10-strip kit is designed to detect Brucella antibodies in whole blood, serum, or plasma.
- The Canine Brucellosis Test Kit is a Rapid Slide Agglutination Test (RSAT) for the diagnosis of Brucella canis that was described by George and Carmichael (Proc. Council Res. Workers in Animal Disease, November 1973). The basis of the test is direct agglutination of the dead antigen stained by Brucella canis antibodies.
Canine brucellosis is a chronic Brucella canis infection that causes generalized lymphadenitis and mild to severe reproductive symptoms. In the male there may be prostatitis, epididymitis, scrotal dermatitis, testicular atrophy, and impotence; Generally, abortion in the last two weeks of gestation, resorption, infertility, and vaginal discharge can be present in the woman. Brucella Canis is infectious to humans. Caution should be exercised when handling the sera to be tested.
The test has been shown to presumptively diagnose Brucella canis infection by detecting a specific antibody that forms between 1 and 4 weeks after infection. The basis for the serodiagnosis of canine brucellosis is the 2-mercaptoethanol tube agglutination test (2ME-TAT) and the rapid slide agglutination test (RSAT). 2ME-TAT and RSAT have shown an excellent correlation in experimentally infected dogs. In field situations, it has been recognized that an occasional healthy dog, culturally negative for Brucella Canis, will react positively on the RSAT, but not on the 2ME-TAT.
The rapid 2-mercaptoethanol slide agglutination test (2ME-RSAT) has been developed in an attempt to eliminate discrepancies between RSAT and 2-ME-TAT. Certain nonspecific agglutinins, which have been reported to be found in normal dog serum, are removed from canine serum when 2-mercaptoethanol is used in 2ME-TAT. Because of this, 2-mercaptoethanol is employed in the modified slip fast agglutination test procedure.
The reagents are used to diagnose suspected Brucella canis infection in dogs. Positive results require additional confirmation.
The antigen and accompanying antiserum have been standardized and must be used together. Store components at 2-7 ° C (35-45 ° C). Do not allow reagents to remain at room temperature for excessive periods of time. Do not freeze.
The serum is required. The test can also be used with whole blood or plasma.