iba-biotagnology

Molecular cloning and characterization of a thermostable and halotolerant endo-β-1,4-glucanase from Microbulbifer sp. ALW1

The bacterium Microbulbifer sp. ALW1 was beforehand characterised with the aptitude to interrupt down the cell wall of brown algae into high-quality items. The organic capabilities of pressure ALW1 have been but to be elucidated. On this research, a gene, particularly MaCel5A, was remoted from the ALW1 pressure genome, encoding an endo-β-1,4-glucanase. MaCel5A was phylogenetically categorized below the glycoside hydrolase household GH5, with the best id to a putative cellulase of Microbulbifer thermotolerans. The recombinant MaCel5A protein purified from heterologous expression in E. coli exhibited most exercise at 50 °C and pH 6.0, respectively, and functioned selectively towards carboxymethyl cellulose and barley β-glucan.

Recombinant MaCel5A demonstrated appreciable tolerance to the publicity to excessive temperature as much as 80 °C for 30 min retaining 49% residual exercise. As well as, MaCel5A confirmed average stability in opposition to pH 5.0-11.Zero and robust stability within the presence of nonionic surfactant. MaCel5A exhibited robust halo-stability and halotolerance. The exercise of the enzyme elevated about tenfold at 0.5 M NaCl, and about fivefold even at 4.Zero M NaCl in comparison with the enzyme exercise with out the addition of salt. The 2 conserved glutamic acid residues in MaCel5A featured the standard catalytic acid/base and nucleophile equipment of glycoside hydrolases. These traits spotlight the commercial utility potential of MaCel5A.

 

Cloning and bodily localization of male-biased repetitive DNA sequences in Spinacia oleracea (Amaranthaceae)

Spinach (Spinacia oleracea Linnaeus, 1753) is a perfect materials for finding out molecular mechanisms of early-stage intercourse chromosome evolution in dioecious crops. Degenerate oligonucleotide-primed polymerase chain response (DOP-PCR) method facilitates the retrotransposon-relevant research by enriching particular repetitive DNA sequences from a micro-dissected single chromosome. We performed genomic subtractive hybridization to display sex-biased DNA sequences through the use of the DOP-PCR amplification merchandise of micro-dissected spinach Y chromosome.

The screening yielded 55 male-biased DNA sequences with 30 576 bp in size, of which, 32 DNA sequences (12 049 bp) contained repeat DNA sequences, together with LTR/CopiaLTR/Gypsy, easy repeats, and DNA/CMC-EnSpm. Amongst these repetitive DNA sequences, 4 DNA sequences that contained a fraction of Ty3-gypsy retrotransposons (SP73, SP75, SP76, and SP77) have been chosen as fluorescence probes to hybridization on female and male spinach karyotypes. Fluorescence in situ hybridization (FISH) alerts of SP73 and SP75 have been captured totally on the centromeres and their surrounding space for every homolog. Hybridization alerts primarily appeared close to the putative centromeres for every homologous chromosome pair through the use of SP76 and SP77 probes for FISH, and sporadic alerts existed on the lengthy arms. Outcomes could be served as a foundation to review the perform of repetitive DNA sequences in intercourse chromosome evolution in spinach.

 

iba-biotagnology
iba-biotagnology

Molecular cloning and purposeful characterization of TaIRI9 gene in wheat (Triticum aestivum L.)

The vernalization of wheat is likely one of the essential elements that decide the planting area, introduction and cultivation strategies of wheat. Nevertheless, the recognized vernalization genes (molecular marker) can’t exactly distinguish the vernalization requirement of winter wheat cultivars. Subsequently, it is very important discover new vernalization genes and elucidate the mechanism of vernalization regulation. To discover the gene community within the vernalization pathway, we screened TaIRI9 (ice recrystallization inhibitor protein) gene related to the expression profile of vernalization remedy of winter wheat Jing 841. Overexpression of TaIRI9 in wild sort wheat resulted in decreased plant top, elevated tiller quantity and delayed heading days.

After 4°C vernalization remedy for 30, 35, 45 or 50 days, TaIRI9 overexpression traces confirmed elevated vernalization requirement and delayed heading time than wild sort, indicating that TaIRI9 could have an effect on vernalization strategy of wheat. As well as, the expression of the TaIRI9 genes have been analyzed in winter Jing 841, robust winter wheat cultivar Xindong 18 and ten recombinant inbred traces (RILs, Hussar x Yanzhan1). The info confirmed that the expression of TaIRI9 was positively related to the requirement of vernalization. These outcomes indicated that TaIRI9 regulates heading and flowering time in wheat by selling VRN2 and inhibiting flowering promoter VRN1 and VRN3 and could also be concerned in wheat vernalization regulation pathway. Bulked segregant CGT-Seq-facilitated map-based cloning of a powdery mildew resistance gene originating from wild emmer wheat (Triticum dicoccoides)

Powdery mildew, attributable to Blumeria graminis f. sp. tritici (Bgt), is a extensively occurring foliar ailments of wheat worldwide. Wild emmer wheat (WEW, Triticum dicoccoides) (AABB, 2n=4x=28), the progenitor of the cultivated tetraploid and hexaploid wheat, is extremely proof against powdery mildew and plenty of resistance alleles have been recognized on this wild species.

Cloning and characterization of a novel DNase gene from Trichogramma pretiosum

DNase is a robust instrument for a sequence of molecular biology purposes. Creating a method for large-scale manufacturing of DNase with excessive purity and exercise is important for scientific analysis. On this research, a beforehand uncharacterized gene with nuclease exercise was present in Trichogramma pretiosum genome. Pichia pastoris GS115 was most popular because the host to beat the problems associated to prokaryotic expression. Underneath the optimum situations, the exercise of T. pretiosum DNase (Tp-DNase) reached 1940 U/mL of tradition supernatant in fed-batch fermentation. Utilizing ion-exchange chromatography and adsorption chromatography, Tp-DNase was produced with a purity of > 99% and molecular weight of 45 kDa.

In vitro DNA degradation experiments confirmed that Tp-DNase may successfully degrade dsDNA, and its exercise was barely larger than that of bovine pancreas DNase I below the identical situations. Furthermore, Tp-DNase can be utilized to remove nucleic acid contamination and enhance the accuracy of nucleic acid detection.

Cloning, expression, and characterization of Baeyer-Villiger monooxygenases from eukaryotic Exophiala jeanselmei pressure KUFI-6N

The fungus Exophiala jeanselmei pressure KUFI-6N produces a novel cycloalkanone monooxygenase (ExCAMO) that shows an unusual substrate spectrum of Baeyer-Villiger oxidation of 4-10-membered ring ketones. On this research, we aimed to establish and sequence the gene encoding ExCAMO from KUFI-6N and overexpress the gene in Escherichia coli. We discovered that the first construction of ExCAMO is most carefully associated to the cycloalkanone monooxygenase from Cylindrocarpon radicicola ATCC 11011, with 54.2% amino acid id. ExCAMO was functionally expressed in Escherichia coli and its substrate spectrum and kinetic parameters investigated.

Substrate profiling indicated that ExCAMO is uncommon amongst recognized Baeyer-Villiger monooxygenases owing to its skill to simply accept quite a lot of substrates, together with C4-C12 membered ring ketones. ExCAMO has excessive affinity and catalytic effectivity towards cycloalkanones, the best being towards cyclohexanone. 5 different genes encoding Baeyer-Villiger monooxygenases have been additionally cloned and expressed in Escherichia coli.

 

 

CD27, Fc fusion

71176 100 µg
EUR 320
Description: Human secreted CD27, Fc fusion protein, also known as Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7, and T14, GenBank Accession No. NM_001242, a.a. 21-192 expressed in a HEK293 cell expression system. MW = 45.8 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

CD47, Fc fusion

71177 100 µg
EUR 325
Description: Human CD47, Fc fusion protein, also known as leukocyte surface antigen CD47, Rh-related antigen, integrin-associated protein, antigenic surface determinant protein OA3, antigen identified by monoclonal antibody 1D8, IAP, and MER6. GenBank Accession No. NM_001777, a.a. 19-139 expressed in a HEK293 cell expression system. MW = 40 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

TIGIT, Fc fusion

71186 100 µg
EUR 320
Description: Human T-cell immunoreceptor with Ig and_x000D_ITIM domains (TIGIT), also known as V-set_x000D_and immunoglobulin domain-containing_x000D_protein 9, VSIG9, V-set and transmembrane_x000D_domain-containing protein 3, and VSTM3,_x000D_GenBank Accession No. NM_173799, a.a._x000D_22-141 fused to Fc region of human IgG,_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 39.7 kDa. This protein runs_x000D_at a higher MW by SDS-PAGE due to_x000D_glycosylation.

Eppendorf Multiporator Helix Fusion Chamber For Cell Fusion - EACH

E4308014008 EACH
EUR 1150.2

Fusion (FUS) Antibody

20-abx110192
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Fusion (FUS) Antibody

20-abx100040
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fusion (FUS) Antibody

20-abx129140
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Fusion (FUS) Antibody

20-abx212423
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  • 100 ul
  • 50 ul

Fusion (FUS) Antibody

20-abx212424
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  • 100 ul
  • 50 ul

Fusion (FUS) Antibody

20-abx172487
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  • 1 mg
  • 200 ug

Fusion (FUS) Antibody

20-abx176518
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  • 1 mg
  • 200 ug

FUS (Fusion) Antibody

E301249 100ug
EUR 275
Description: Available in various conjugation types.

FUS (Fusion) Antibody

E301250 100ug/200ul
EUR 275
Description: Available in various conjugation types.

Morpheus Fusion FX

M-MD1-130-FX 96 x 100 ul ul
EUR 67
Description: Morpheus Fusion FX

Recombinant Fusion (FUS)

RPC260Hu01 10ug
EUR 164

Recombinant Fusion (FUS)

4-RPC260Hu01
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  • 5 mg
Description: Recombinant Human Fusion expressed in: E.coli

Recombinant Fusion (FUS)

RPC260Mu01 10ug
EUR 180

Recombinant Fusion (FUS)

4-RPC260Mu01
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  • 100 ug
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  • 5 mg
Description: Recombinant Mouse Fusion expressed in: E.coli

Recombinant Fusion (FUS)

RPC260Mu02 10ug
EUR 140

Recombinant Fusion (FUS)

RPU57089-100ug 100ug
EUR 470.4

Recombinant Fusion (FUS)

RPU57089-1mg 1mg
EUR 2184

Recombinant Fusion (FUS)

RPU57089-50ug 50ug
EUR 385

Recombinant Fusion (FUS)

RPU51790-100ug 100ug
EUR 504.9

Recombinant Fusion (FUS)

RPU51790-1mg 1mg
EUR 2238.6

Recombinant Fusion (FUS)

RPU51790-50ug 50ug
EUR 405.9

Recombinant Fusion (FUS)

RPU41541-100ug 100ug
EUR 554.4

Recombinant Fusion (FUS)

RPU41541-1mg 1mg
EUR 2457

Recombinant Fusion (FUS)

RPU41541-50ug 50ug
EUR 445.5

TIM-3, Fc fusion

71151 100 µg
EUR 320
Description: Human secreted TIM-3, Fc fusion protein, also known as T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, T-cell and immunoglobulin and mucin domain-containing protein 3, TIMD-3, Hepatitis A virus cellular receptor 2, and HAVCR-2. GenBank Accession No. NM_032782.4, a.a. 22-200 expressed in a HEK293 cell expression system. MW = 46.5 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Fusion glycoprotein F0

E8ER1802-33 100ul
EUR 275
Description: Available in various conjugation types.

Fusion glycoprotein F0

E8ER1803-51 100ul
EUR 275
Description: Available in various conjugation types.

Human LTBR, Fc fusion

71112 100 µg
EUR 310
Description: Human secreted lymphotoxin beta receptor_x000D_(LTBR)-Fc fusion protein, also known as_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_002342, a.a 28-219, expressed in_x000D_a HEK293 cell expression system. MW = 48_x000D_kDa (monomer). This protein runs at a_x000D_higher apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

Mouse LTBR, Fc fusion

71122 100 µg
EUR 340
Description: Mouse secreted lymphotoxin beta receptor_x000D_(mLTBR)-Fc fusion protein, also known as,_x000D_Tumor Necrosis Factor C Receptor_x000D_(TNFCR), and CD18, GenBank Accession_x000D_No. NM_010736, a.a 28-221, expressed in_x000D_a HEK293 cell expression system. MW =_x000D_48.8 kDa (monomer). Endotoxin level_x000D_<0.001 EU/ug.This protein runs at a higher_x000D_apparent M.W. by SDS-PAGE due to_x000D_glycosylation.

BTLA(CD272), Fc fusion

71141 100 µg
EUR 320
Description: Human B- and T-lymphocyte attenuator (BTLA)-Fc fusion protein, also known as CD272, GenBank Accession No. NM_181780, a.a. 31-150, expressed in a HEK293 cell expression system. MW = 40.3 kDa (monomer). This protein runs at a higher apparent M.W. by SDS-PAGE due to glycosylation.

LAG3 (CD223), Fc fusion

71146 100 µg
EUR 320
Description: Human secreted LAG3, Fc fusion protein, also known as Lymphocyte-Activation Gene 3 and CD223. GenBank Accession No. NM_002286, a.a. 23-450 expressed in a HEK293 cell expression system. MW = 73.1 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

GITR (CD357), Fc fusion

71172 100 µg
EUR 300
Description: Human secreted GITR, Fc fusion protein, also known as Glucocorticoid-induced TNFR-Related Protein, Tumor Necrosis Factor Receptor Superfamily member 18, TNFRSF18, Activation-Inducible TNFR Family Receptor, AITR, and CD357, GenBank Accession No. NM_004195, a.a. 26-161 expressed in a HEK293 cell expression system. MW = 41.2 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.

OX40 (CD134), Fc fusion

71175 100 µg
EUR 320
Description: Human secreted OX40, Fc fusion protein,_x000D_also known as Tumor Necrosis Factor_x000D_Receptor Superfamily Member 4,_x000D_TNFRSF4, and CD134, GenBank_x000D_Accession No. NM_003327, a.a. 29-216_x000D_expressed in a HEK293 cell expression_x000D_system. MW = 46.8 kDa (monomer). This_x000D_protein runs at a higher MW by SDS-PAGE_x000D_due to glycosylation.

ICOS (CD278), Fc fusion

71179 100 µg
EUR 320
Description: Human ICOS, Fc fusion protein, also known as inducible T-cell costimulator and CD antigen 278, activation-inducible lymphocyte immunomediatory molecule, AILIM, CD278, and CVID1. GenBank Accession No. NM_012092, a.a. 21-140 expressed in a HEK293 cell expression system. MW = 40.2 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.

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