Single Copy Oligonucleotide Fluorescence In Situ Hybridization Probe Design Platforms: Development, Application and Evaluation
Gerbil / Goat / Guinea Pig / Hamster / Helicobacter / Purification / purified / Rabbit / Real-time / Recombinant

Single Copy Oligonucleotide Fluorescence In Situ Hybridization Probe Design Platforms: Development, Application and Evaluation

- August 24, 2021 | August 25, 2021 - Johnny

Oligonucleotides fluorescence in situ hybridization (Oligo-FISH) is an rising know-how and is a crucial device in analysis areas comparable to detection of chromosome variation, identification of allopolyploid, and deciphering of three-dimensional (3D) genome constructions. Primarily based on the demand for extremely environment friendly oligo probes for oligo-FISH experiments, growing numbers of instruments have been developed for probe design lately. Out of date oligonucleotide design instruments have been tailored for oligo-FISH probe design due to their comparable issues.

With the event of DNA sequencing and large-scale synthesis, novel instruments have been designed to extend the specificity of designed oligo probes and allow genome-scale oligo probe design, which has tremendously improved the appliance of single copy oligo-FISH. Regardless of this, few research have launched the event of the oligo-FISH probe design instruments and their utility in FISH experiments systematically. Apart from, a complete comparability and analysis is missing for the out there instruments. On this evaluation, we offer an outline of the oligo-FISH probe design course of, summarize the event and utility of the out there instruments, consider a number of state-of-art instruments, and finally present steering for single copy oligo-FISH probe design.

At the moment there’s an pressing must develop antiviral medicine and alleviate present COVID-19 pandemic. Though many candidates have been developed, we right here designed and constructed chimeric oligonucleotides comprising a 2′-OMe modified antisense oligonucleotide and a 5′-phosphorylated 2′-5′ poly(A) 4 (4A 2-5 ) to degrade envelope and spike RNAs of SARS-CoV-2. The oligonucleotide was used for looking and recognizing goal viral RNA sequence, and the conjugated 4A 2-5 was used for guided ribonuclease L (RNase L) activation to sequence-specifically degrade viral RNAs.

Since RNase L can potently cleave single strand RNA throughout innate antiviral response, degradation efficiencies with these chimeras have been twice as a lot as solely 2′-OMe modified oligonucleotides for each SARS-CoV-2 RNA targets. In pseudovirus an infection fashions, one of many chimeras concentrating on spike RNA achieved potent and broad-spectrum inhibition of SARS-CoV-2 and its N501Y and/or ΔH69/ΔV70 mutants.

Simultaneous quantitation of inorganic ions in oligonucleotides utilizing mixed-mode liquid chromatography coupled with a charged aerosol detector

The quantities of inorganic ions (e.g. sodium, potassium, magnesium, calcium, chloride, and phosphate) in intrathecally administered oligonucleotide drug merchandise should be managed with a view to meet the ultimate goal formulation composition. Throughout course of growth, these ions are measured to make sure the answer composition doesn’t change throughout purification or drug product compounding and to make sure the ultimate drug product targets are achieved.

A quantitative methodology for ion evaluation in antisense oligonucleotide (ASO) medicine was developed by mixed-mode anion trade/cation exchange-hydrophilic interplay liquid chromatography (AEX/CEX-HILIC) with a charged aerosol detector (CAD). This thirteen-minute methodology measures monovalent and multivalent cations and anions concurrently. LC separation circumstances, CAD parameters, and pattern preparation have been judiciously optimized to make sure that the strategy is restricted, correct, exact, linear, strong, and rugged. With this LC-CAD method, ion evaluation for oligonucleotide medicine may be carried out in most analytical labs to help drug growth.

Single Copy Oligonucleotide Fluorescence In Situ Hybridization Probe Design Platforms: Development, Application and Evaluation

Antisense Oligonucleotide-Mediated Discount of HDAC6 Does Not Scale back Tau Pathology in P301S Tau Transgenic Mice

Acetylation of tau protein is dysregulated in Alzheimer’s Illness (AD). It has been proposed that acetylation of particular websites within the KXGS motif of tau can regulate phosphorylation of close by residues and scale back the propensity of tau to mixture. Histone deacetylase 6 (HDAC6) is a cytoplasmic enzyme concerned in deacetylation of a number of targets, together with tau, and it has been steered that inhibition of HDAC6 would enhance tau acetylation on the KXGS motifs and thus might current a viable therapeutic method to deal with AD.

To instantly take a look at the contribution of HDAC6 to tau pathology, we intracerebroventricularly injected an antisense oligonucleotide (ASO) directed towards HDAC6 mRNA into brains of P301S tau mice (PS19 mannequin), which resulted in a 70% knockdown of HDAC6 protein within the mind. Regardless of a sturdy lower in ranges of HDAC6, no enhance in tau acetylation was noticed. Moreover, no change of tau phosphorylation or tau aggregation was detected upon the knockdown of HDAC6. We conclude that HDAC6 doesn’t affect tau pathology in PS19 mice.

Efficient silencing of miR-126 after ischemic stroke via intravenous α-tocopherol-conjugated heteroduplex oligonucleotide in mice

Mind endothelial cells (BECs) are concerned within the pathogenesis of ischemic stroke. Lately, a number of microRNAs (miRNAs) in BECs have been reported to manage the endothelial operate in ischemic mind. Due to this fact, modulation of miRNAs in BECs by a therapeutic oligonucleotide to inhibit miRNA (antimiR) might be a helpful technique for treating ischemic stroke. Nevertheless, few makes an attempt have been made to realize this technique through systemic route because of lack of environment friendly delivery-method towards BECs.

Right here, we have now developed a brand new know-how for delivering an antimiR into BECs and silencing miRNAs in BECs, utilizing a mouse ischemic stroke mannequin. We designed a heteroduplex oligonucleotide, comprising an antimiR towards miRNA-126 (miR-126) often called the endothelial-specific miRNA and its complementary RNA, conjugated to α-tocopherol as a supply ligand (Toc-HDO concentrating on miR-126). Intravenous administration of Toc-HDO concentrating on miR-126 remarkably suppressed miR-126 expression in ischemic mind of the mannequin mice.

FAM129B Antibody
MBS9629964-5x02mL 5x0.2mL
EUR 1220

FAM129B Antibody
MBS9632347-1mg 1mg
EUR 375

FAM129B Antibody
MBS9632347-5x1mg 5x1mg
EUR 1545

FAM129B polyclonal antibody
BS77935 50ul
EUR 358
Description: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

FAM129B Polyclonal Antibody
E-AB-92187-120uL 120uL
EUR 320
Description: Unconjugated

FAM129B Polyclonal Antibody
E-AB-92187-200uL 200uL
EUR 530
Description: Unconjugated

FAM129B Polyclonal Antibody
E-AB-92187-60uL 60uL
EUR 200
Description: Unconjugated

FAM129B Polyclonal Antibody
E-AB-92187-each each Ask for price
Description: Unconjugated

FAM129B Polyclonal Antibody
MBS9238489-01mL 0.1mL
EUR 415

FAM129B Polyclonal Antibody
MBS9238489-5x01mL 5x0.1mL
EUR 1841

FAM129B (Ab-712) Antibody
CSB-PA998601- each
EUR 402
Description: A polyclonal antibody against FAM129B (Ab-712). Recognizes FAM129B (Ab-712) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

FAM129B (Ab-712) Antibody
CSB-PA998601-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against FAM129B (Ab-712). Recognizes FAM129B (Ab-712) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IF;WB:1:500-1:1000, IF:1:100-1:200

FAM129B (Ab-712) Antibody
MBS7132002-01mL 0.1mL
EUR 270

FAM129B (Ab-712) Antibody
MBS7132002-5x01mL 5x0.1mL
EUR 1200

Anti-Niban-like protein(Ab-712) FAM129B Antibody
A09391 100ul
EUR 360
Description: Boster Bio Anti-Niban-like protein(Ab-712) FAM129B Antibody (Catalog # A09391). Tested in WB, IF applications. This antibody reacts with Human, Mouse.

FAM129B antibody - C-terminal region
MBS3215723-01mL 0.1mL
EUR 455

FAM129B antibody - C-terminal region
MBS3215723-5x01mL 5x0.1mL
EUR 1995

Rabbit Anti-Human FAM129B (aa 710-714) Polyclonal Antibody
CPB-1199RH 100 ul
EUR 414.96
Description: Rabbit

FAM129B Antibody, Rabbit PAb, Antigen Affinity Purified
MBS8124415-01mL 0.1mL
EUR 300

FAM129B Antibody, Rabbit PAb, Antigen Affinity Purified
MBS8124415-5x01mL 5x0.1mL
EUR 1200

ARP62735_P050-25UL - FAM129B Antibody - C-terminal region
ARP62735_P050-25UL 25ul
EUR 99

Anti-Phospho-Ser679/683 FAM129B
MBS502226-01mL 0.1mL
EUR 435

Anti-Phospho-Ser679/683 FAM129B
MBS502226-5x01mL 5x0.1mL
EUR 1805

ARP62735_P050 - FAM129B antibody - C-terminal region (ARP62735_P050)
ARP62735_P050 100ul
EUR 389

Fam129b/ Rat Fam129b ELISA Kit
ELI-45712r 96 Tests
EUR 1063.2

Family with Sequence Similarity 129, Member B (FAM129B) Antibody
abx331409-100ul 100 ul
EUR 510

Family With Sequence Similarity 129, Member B (FAM129B) Antibody
abx232965-100ug 100 ug
EUR 610.8

FAM129B siRNA (Rat)
MBS8237891-15nmol 15nmol
EUR 405

FAM129B siRNA (Rat)
MBS8237891-30nmol 30nmol
EUR 565

FAM129B siRNA (Rat)
MBS8237891-5x30nmol 5x30nmol
EUR 2450

FAM129B Rabbit pAb
A20173 500μL
EUR 262.15

FAM129B Rabbit pAb
E45R29195N 50 ul
EUR 297.75

FAM129B Rabbit pAb
MBS9143585-002mL 0.02mL
EUR 200

FAM129B Rabbit pAb
MBS9143585-005mL 0.05mL
EUR 255

FAM129B Rabbit pAb
MBS9143585-01mL 0.1mL
EUR 345

FAM129B Rabbit pAb
MBS9143585-02mL 0.2mL
EUR 545

FAM129B Rabbit pAb
MBS9143585-5x02mL 5x0.2mL
EUR 2265

FAM129B siRNA (Mouse)
MBS829302-15nmol 15nmol
EUR 405

FAM129B siRNA (Mouse)
MBS829302-30nmol 30nmol
EUR 565

FAM129B siRNA (Mouse)
MBS829302-5x30nmol 5x30nmol
EUR 2450

FAM129B siRNA (Human)
MBS828174-15nmol 15nmol
EUR 405

FAM129B siRNA (Human)
MBS828174-30nmol 30nmol
EUR 565

FAM129B siRNA (Human)
MBS828174-5x30nmol 5x30nmol
EUR 2450

FAM129B ELISA KIT|Human
EF009517 96 Tests
EUR 826.8

Rat FAM129B shRNA Plasmid
20-abx990325
  • Ask for price
  • Ask for price
  • 150 µg
  • 300 µg

Human FAM129B Protein Lysate
MBS8411398-002mg 0.02mg
EUR 545

Human FAM129B Protein Lysate
MBS8411398-5x002mg 5x0.02mg
EUR 2225

Fam129b (NM_146119) Mouse Recombinant Protein
E45M45119M5 20 ug
EUR 1924.54

FAM129B Recombinant Protein (Rat)
RP200402 100 ug Ask for price

FAM129B Recombinant Protein (Human)
RP057474 100 ug Ask for price

FAM129B Recombinant Protein (Mouse)
RP132935 100 ug Ask for price

Fam129b (untagged) - Mouse family with sequence similarity 129, member B (Fam129b), (10ug)
MC203028 10 µg Ask for price

anti- FAM160B1 antibody
FNab02970 100µg
EUR 702
Description: Antibody raised against FAM160B1

anti- FAM160B2 antibody
FNab02971 100µg
EUR 702
Description: Antibody raised against FAM160B2

anti- FAM111B antibody
FNab02954 100µg
EUR 702
Description: Antibody raised against FAM111B

anti- FAM116B antibody
FNab02956 100µg
EUR 702
Description: Antibody raised against FAM116B

anti- FAM117B antibody
FNab02957 100µg
EUR 702
Description: Antibody raised against FAM117B

anti- FAM118B antibody
FNab02958 100µg
EUR 658.5
Description: Antibody raised against FAM118B

anti- FAM124B antibody
FNab02961 100µg
EUR 606.3
Description: Antibody raised against FAM124B

anti- FAM127B antibody
FNab02963 100µg
EUR 658.5
Description: Antibody raised against FAM127B

anti- FAM168B antibody
FNab02973 100µg
EUR 702
Description: Antibody raised against FAM168B

anti- FAM186B antibody
FNab02976 100µg
EUR 702
Description: Antibody raised against FAM186B

anti- FAM40B antibody
FNab02984 100µg
EUR 658.5
Description: Antibody raised against FAM40B

anti- FAM46B antibody
FNab02985 100µg
EUR 702
Description: Antibody raised against FAM46B

anti- FAM49B antibody
FNab02987 100µg
EUR 658.5
Description: Antibody raised against FAM49B

anti- FAM57B antibody
FNab02989 100µg
EUR 658.5
Description: Antibody raised against FAM57B

anti- FAM65B antibody
FNab02991 100µg
EUR 658.5
Description: Antibody raised against FAM65B

anti- FAM84B antibody
FNab02997 100µg
EUR 702
Description: Antibody raised against FAM84B

anti- FAM96B antibody
FNab03000 100µg
EUR 658.5
Description: Antibody raised against FAM96B

anti- FAM98B antibody
FNab03001 100µg
EUR 658.5
Description: Antibody raised against FAM98B

Fam129b ORF Vector (Rat) (pORF)
ORF066802 1.0 ug DNA
EUR 607.2

Fam129b (Myc-DDK-tagged) - Mouse family with sequence similarity 129, member B (Fam129b)
MR210452 10 µg Ask for price

FAM129B ORF Vector (Human) (pORF)
ORF019159 1.0 ug DNA
EUR 486

Fam129b ORF Vector (Mouse) (pORF)
ORF044313 1.0 ug DNA
EUR 607.2

Human FAM129B knockout cell line
ABC-KH5089 1 vial Ask for price
Description: Human FAM129B knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human FAM129B knockdown cell line
ABC-KD5089 1 vial Ask for price
Description: Human FAM129B knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human FAM129B Protein Lysate 20ug
IHUFAM129BPLLY20UG each
EUR 361
Description: Human FAM129B Protein Lysate 20ug

Fam129b (untagged ORF) - Rat family with sequence similarity 129, member B (Fam129b), (10 ug)
RN215551 10 µg Ask for price

FAM129B Peptide - C-terminal region
MBS3240626-01mg 0.1mg
EUR 180

FAM129B Peptide - C-terminal region
MBS3240626-5x01mg 5x0.1mg
EUR 730

OCOA08502-20UG - FAM129B Protein Lysate
OCOA08502-20UG 20ug
EUR 269

FAM129B (untagged)-Human family with sequence similarity 129, member B (FAM129B), transcript variant 1
SC305057 10 µg Ask for price

FAM129B (untagged)-Human family with sequence similarity 129, member B (FAM129B), transcript variant 2
SC302844 10 µg Ask for price

Anti-NSE2 FAM84B Antibody
A13540-1 100ul
EUR 380
Description: Boster Bio Anti-NSE2 FAM84B Antibody catalog # A13540-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human.

Anti-FAM129B antibody
PAab02965 100 ug
EUR 463.2

FAM129B (GFP-tagged) - Human family with sequence similarity 129, member B (FAM129B), transcript variant 2
RG221268 10 µg Ask for price

FAM129B (GFP-tagged) - Human family with sequence similarity 129, member B (FAM129B), transcript variant 1
RG220901 10 µg Ask for price

Anti-Protein FAM3B FAM3B Antibody
A07603 100ul
EUR 380
Description: Boster Bio Anti-Protein FAM3B FAM3B Antibody catalog # A07603. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Fam129b (Myc-DDK-tagged ORF) - Rat family with sequence similarity 129, member B (Fam129b), (10 ug)
RR215551 10 µg Ask for price

Fam129b sgRNA CRISPR Lentivector set (Rat)
K6504001 3 x 1.0 ug
EUR 406.8

Lenti ORF clone of Fam129b (mGFP-tagged) - Mouse family with sequence similarity 129, member B (Fam129b)
MR210452L4 10 µg Ask for price

Fam129b sgRNA CRISPR Lentivector set (Mouse)
K4870701 3 x 1.0 ug
EUR 406.8

FAM129B sgRNA CRISPR Lentivector set (Human)
K0745701 3 x 1.0 ug
EUR 406.8

FAM129B (Myc-DDK-tagged)-Human family with sequence similarity 129, member B (FAM129B), transcript variant 2
RC221268 10 µg Ask for price

FAM129B (Myc-DDK-tagged)-Human family with sequence similarity 129, member B (FAM129B), transcript variant 1
RC220901 10 µg Ask for price

Fam129b 3'UTR GFP Stable Cell Line
TU156140 1.0 ml Ask for price

FAM129B 3'UTR GFP Stable Cell Line
TU057555 1.0 ml
EUR 1825.2

Fam129b 3'UTR GFP Stable Cell Line
TU254268 1.0 ml Ask for price

Lenti ORF clone of Fam129b (Myc-DDK-tagged) - Mouse family with sequence similarity 129, member B (Fam129b)
MR210452L3 10 µg Ask for price

Anti-RAI16/FAM160B2 Antibody, Rabbit Polyclonal
MBS8108965-01mL 0.1mL
EUR 300

Anti-RAI16/FAM160B2 Antibody, Rabbit Polyclonal
MBS8108965-5x01mL 5x0.1mL
EUR 1200

FAM129B Protein Vector (Rat) (pPM-C-HA)
PV267208 500 ng
EUR 1399.2

FAM129B Protein Vector (Rat) (pPB-C-His)
PV267206 500 ng
EUR 1399.2

FAM129B Protein Vector (Rat) (pPB-N-His)
PV267207 500 ng
EUR 1399.2

FAM129B Protein Vector (Rat) (pPM-C-His)
PV267209 500 ng
EUR 1399.2

FAM129B Protein Vector (Mouse) (pPM-C-HA)
PV177252 500 ng
EUR 1278

FAM129B Protein Vector (Human) (pPM-C-HA)
PV076635 500 ng
EUR 662.4

FAM129B Protein Vector (Mouse) (pPB-C-His)
PV177250 500 ng
EUR 1278

FAM129B Protein Vector (Mouse) (pPB-N-His)
PV177251 500 ng
EUR 1278

FAM129B Protein Vector (Mouse) (pPM-C-His)
PV177253 500 ng
EUR 1278

FAM129B Protein Vector (Human) (pPB-C-His)
PV076633 500 ng
EUR 662.4

FAM129B Protein Vector (Human) (pPB-N-His)
PV076634 500 ng
EUR 662.4

FAM129B Protein Vector (Human) (pPM-C-His)
PV076636 500 ng
EUR 662.4

Fam129b 3'UTR Luciferase Stable Cell Line
TU106140 1.0 ml Ask for price

Fam129b 3'UTR Luciferase Stable Cell Line
TU204268 1.0 ml Ask for price

FAM129B 3'UTR Luciferase Stable Cell Line
TU007555 1.0 ml
EUR 1825.2

Fam129b sgRNA CRISPR Lentivector (Rat) (Target 1)
K6504002 1.0 ug DNA
EUR 184.8

Fam129b sgRNA CRISPR Lentivector (Rat) (Target 2)
K6504003 1.0 ug DNA
EUR 184.8

Fam129b sgRNA CRISPR Lentivector (Rat) (Target 3)
K6504004 1.0 ug DNA
EUR 184.8

Recombinant Rat Niban-like protein 1 (Fam129b)
MBS1168872-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1595

Recombinant Rat Niban-like protein 1 (Fam129b)
MBS1168872-002mgEColi 0.02mg(E-Coli)
EUR 1330

Recombinant Rat Niban-like protein 1 (Fam129b)
MBS1168872-002mgYeast 0.02mg(Yeast)
EUR 1355

Recombinant Rat Niban-like protein 1 (Fam129b)
MBS1168872-01mgEColi 0.1mg(E-Coli)
EUR 1600

Recombinant Rat Niban-like protein 1 (Fam129b)
MBS1168872-01mgYeast 0.1mg(Yeast)
EUR 1595

Fam129b sgRNA CRISPR Lentivector (Mouse) (Target 1)
K4870702 1.0 ug DNA
EUR 184.8

Fam129b sgRNA CRISPR Lentivector (Mouse) (Target 2)
K4870703 1.0 ug DNA
EUR 184.8

Fam129b sgRNA CRISPR Lentivector (Mouse) (Target 3)
K4870704 1.0 ug DNA
EUR 184.8

FAM129B sgRNA CRISPR Lentivector (Human) (Target 1)
K0745702 1.0 ug DNA
EUR 184.8

FAM129B sgRNA CRISPR Lentivector (Human) (Target 2)
K0745703 1.0 ug DNA
EUR 184.8

FAM129B sgRNA CRISPR Lentivector (Human) (Target 3)
K0745704 1.0 ug DNA
EUR 184.8

Recombinant Human Niban-like protein 1 (FAM129B)
MBS1345075-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1595

Recombinant Human Niban-like protein 1 (FAM129B)
MBS1345075-002mgEColi 0.02mg(E-Coli)
EUR 1325

Recombinant Human Niban-like protein 1 (FAM129B)
MBS1345075-002mgYeast 0.02mg(Yeast)
EUR 1355

Recombinant Human Niban-like protein 1 (FAM129B)
MBS1345075-01mgEColi 0.1mg(E-Coli)
EUR 1600

Recombinant Human Niban-like protein 1 (FAM129B)
MBS1345075-01mgYeast 0.1mg(Yeast)
EUR 1590

Recombinant Mouse Niban-like protein 1 (Fam129b)
MBS1375407-002mgBaculovirus 0.02mg(Baculovirus)
EUR 1595

Recombinant Mouse Niban-like protein 1 (Fam129b)
MBS1375407-002mgEColi 0.02mg(E-Coli)
EUR 1330

Recombinant Mouse Niban-like protein 1 (Fam129b)
MBS1375407-002mgYeast 0.02mg(Yeast)
EUR 1355

Recombinant Mouse Niban-like protein 1 (Fam129b)
MBS1375407-01mgEColi 0.1mg(E-Coli)
EUR 1605

Recombinant Mouse Niban-like protein 1 (Fam129b)
MBS1375407-01mgYeast 0.1mg(Yeast)
EUR 1595

Lenti ORF particles, Fam129b (GFP-tagged) - Mouse family with sequence similarity 129, member B (Fam129b), 200ul, >10^7 TU/mL
MR210452L4V 200 µl Ask for price

Lenti ORF clone of Fam129b (mGFP-tagged ORF) - Rat family with sequence similarity 129, member B (Fam129b), (10 ug)
RR215551L4 10 µg Ask for price

AAP62735-100UG - FAM129B Peptide - C-terminal region
AAP62735-100UG 100ug
EUR 99

Lenti ORF particles, Fam129b (GFP-tagged ORF) - Rat family with sequence similarity 129, member B (Fam129b), 200ul, >10^7 TU/mL
RR215551L4V 200 µl Ask for price

As well as, we confirmed that Toc-HDO concentrating on miR-126 was delivered into BECs extra effectively than the guardian antimiR in ischemic mind, and that it was delivered extra successfully in ischemic mind than non-ischemic mind of this mannequin mice. Our research highlights the potential of this know-how as a brand new medical therapeutic possibility for ischemic stroke.

Leave a Reply

Your email address will not be published. Required fields are marked *